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Infection Control · single question

Per CDC guidelines, autoclave biological spore testing should be performed at what minimum frequency?

  1. With each sterilization cycle
  2. Monthly only
  3. At least weekly (with every load containing implantable items)
  4. Daily
Show answer and explanation

Correct answer: C. At least weekly (with every load containing implantable items)

  • A. Testing with each individual cycle is not required for non-implantable items.
  • B. Monthly testing alone is insufficient per CDC standards — weekly is the minimum.
  • C. Correct. The CDC recommends at least WEEKLY biological monitoring of heat sterilizers (autoclaves) using Geobacillus stearothermophilus spore strips/vials. Additionally, biological monitoring should be performed with EVERY load containing implantable items (items placed in tissue/bone). Defective pouches or failed spore tests require removing the sterilizer from service.
  • D. Daily testing is not required by current CDC guidelines (it would be impractical and cost-prohibitive).

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