Infection Control · single question

Regarding spore testing for sterilization monitoring: all of the following are TRUE EXCEPT:

  1. Biological indicators (spore tests) use Geobacillus stearothermophilus spores for steam autoclaves
  2. Spore tests should be performed weekly per CDC guidelines
  3. Spore tests should be performed MONTHLY
  4. Results should be recorded and maintained for tracking
Show answer and explanation

Correct answer: C. Spore tests should be performed MONTHLY

The CDC recommends weekly biological spore testing (not monthly) for steam autoclaves in dental settings. OSHA and most state dental boards also require at minimum weekly spore testing. Monthly testing is insufficient and represents the FALSE/exception statement. Biological indicators containing heat-resistant bacterial spores (Geobacillus stearothermophilus for steam, Bacillus atrophaeus for dry heat) are the gold standard for verifying sterilization efficacy.

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