Micro & Immunology · single question

Geobacillus stearothermophilus, used as a biological indicator for autoclave (steam) sterilization, is:

  1. Highly resistant and pathogenic
  2. Less resistant and non-pathogenic
  3. Less resistant and pathogenic
  4. Highly resistant and non-pathogenic
Show answer and explanation

Correct answer: D. Highly resistant and non-pathogenic

  • A. Incorrect – it is not a human pathogen.
  • B. Incorrect – low resistance would not validate the cycle.
  • C. Incorrect – a biological indicator must be HIGHLY resistant to be meaningful.
  • D. Correct – its spores are highly heat-resistant (so killing them proves sterilization) yet it is non-pathogenic (safe to handle as a test organism).

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