Pathology · single question
An older woman with pemphigus vulgaris presents with desquamative gingivitis. Which biopsy technique is appropriate?
Show answer and explanation
Correct answer: A. Perilesional biopsy (intact mucosa adjacent to the lesion) for direct immunofluorescence
- A. Correct. For vesiculobullous/autoimmune disease, take a perilesional specimen (and send for direct immunofluorescence) because the diagnostic intact epithelium is at the margin, not the sloughed center.
- B. The ulcer center has lost epithelium and is non-diagnostic.
- C. Cytology cannot demonstrate the immunofluorescence pattern needed for diagnosis.
- D. Biopsy with DIF is essential to confirm pemphigus.