Infection Control · single question

Which of the following statements about biological spore testing (autoclave sterilization monitoring) is NOT true?

  1. Spore tests should be performed at least weekly
  2. At least weekly testing is recommended by the CDC for dental offices
  3. Spore testing should be done each day the autoclave is used
  4. Positive spore test results indicate a sterilization failure requiring immediate action
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Correct answer: C. Spore testing should be done each day the autoclave is used

The CDC and ADA recommend that dental offices perform biological indicator (spore) testing at least weekly — not necessarily every day the sterilizer is used. Biological monitoring using Geobacillus stearothermophilus spore strips or ampoules is the gold standard for verifying sterilizer performance. A positive spore test after a sterilization cycle indicates a malfunction; the sterilizer should be taken out of service, the cause identified and corrected, and the test repeated before returning to clinical use. In-office dental sterilizers should be tested weekly.

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