Infection Control · single question

What type of test is the autoclave spore test (biological indicator)?

  1. Mechanical monitoring test
  2. Liquid chemical sterilization test
  3. Chemical indicator test
  4. Heat sterilization test (biological indicator)
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Correct answer: D. Heat sterilization test (biological indicator)

  • A. Mechanical monitoring uses gauges and printouts — not biological spore tests.
  • B. Liquid chemical sterilization (cold sterilization) uses different monitoring methods.
  • C. Chemical indicators change color based on temperature or chemical exposure — they are class 1-6 chemical monitors, not biological indicators.
  • D. Correct. The autoclave spore test IS a biological indicator test using heat-resistant bacterial spores (Geobacillus stearothermophilus for steam autoclaves, Bacillus atrophaeus for dry heat/chemical vapor). If sterilization is adequate, the spores are killed. This is the GOLD STANDARD (most reliable) test for sterilization efficacy. It should be done WEEKLY.

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