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Infection Control · single question

What is NOT true about biological spore tests for autoclaves?

  1. A positive spore test indicates sterilization failure
  2. They should be run only once a month
  3. They verify sterilization effectiveness
  4. They should be run at least weekly
Show answer and explanation

Correct answer: B. They should be run only once a month

  • A. Incorrect — this IS true. A positive spore test means sterilization FAILED; all items in that load must be considered non-sterile.
  • B. Correct — NOT true. Running spore tests only once a MONTH is INSUFFICIENT. CDC and OSAP guidelines recommend at least WEEKLY spore testing. Once monthly does not meet quality assurance standards.
  • C. Incorrect — this IS true. Biological indicators (Geobacillus stearothermophilus spores) are the gold standard for autoclave verification.
  • D. Incorrect — this IS true (weekly or more is the correct frequency).

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